Usyk dropped Dubois with a short right hand just over one minute into the fifth round. He then closed the show with a thunderous left hook, cementing his place as one of the greatest heavyweights ever.
The 38-year-old Ukrainian remains unbeaten and adds the IBF world heavyweight title to his collection. Many fans and analysts now consider Usyk one of the best heavyweights of all time.
After the fight, Usyk faced off with YouTuber-turned-boxer Jake Paul in the ring. This encounter sparked speculation about a potential matchup between the two.
Usyk's manager, Egis Klimas, addressed these rumors in an interview with Boxing King Media. He made it clear that a boxing match between Usyk and Paul won't happen.
"Well Jake Paul, you know, when it comes to Jake Paul we're not talking about the boxing because we would be probably put in the jail for murder … we all understand we can't put Jake Paul and Oleksandr in the ring for boxing, there is no way, and Oleksandr will never do that"
However, Klimas didn't completely rule out the possibility of the two facing each other. He suggested an MMA contest could work, given Paul's wrestling background.
"but of course because Jake Paul was a wrestler and what I heard he was a good wrestler when he was growing up, he knows how to wrestle, he knows how to fight a little bit. I think MMA would be the perfect thing to do."
It's uncertain whether Usyk will continue his boxing career after this victory. For now, the Ukrainian great can bask in the glory of his historic achievement at Wembley Stadium.
You can find more details about Usyk's victory over Dubois on the BBC Sport website.